Equilibrium intermediates in the unfolding pathway of creatine kinase |
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Authors: | YL Zhang YX Fan GC Huang JX Zhou JM Zhou |
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Affiliation: | Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520-8002, USA. |
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Abstract: | The unfolding of creatine kinase in various concentrations of guanidine hydrochloride of increasing concentrations has been investigated by combination of size-exclusion chromatography (SEC) with other methods. There are two peaks in the profiles of SEC in GuHCl at moderate concentrations, showing that unfolding of creatine kinase goes through dimeric and monomeric intermediates with increasing guanidine hydrochloride concentrations. Both intermediates have relatively compact structure and retain considerable ordered structure. |
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