酶解反应与膜分离耦合连续制备酪蛋白磷酸肽

采用酶解反应与膜分离耦合新工艺连续水解全酪蛋白制备酪蛋白磷酸肽(CPPs)。考察了超滤膜对胰蛋白酶及底物溶液的截留效果;研究了初始底物质量浓度、初始酶质量浓度、反应体积、膜渗透通量等参数对反应器性能和反应转化率的影响规律;利用高效凝胶排阻色谱系统(HPSEC)对酶解产物进行检测分析;建立了酶膜反应器连续水解动力学模型,并对间歇与连续酶解过程进行比较分析,证明反应-分离耦合技术可使酶解效率及蛋白酶利用率大幅提高,并使产物得到调控与富集,为CPPs的酶法制备提供了一种更为有效的方法。

Continuous preparation of casein phosphopeptides by integration of enzymatic hydrolysis and membrane separation

Casein phosphopeptides(CPPs) were continuously prepared by coupling of enzymatic hydrolysis and membrane separation.In order to prepare target active peptides with high purity and good yield,operating stability of trypsin and membrane rejection of casein were studied respectively.The influences of operating parameters,including substrate mass concentration,enzyme mass concentration,reaction volume and permeation flux,on the reaction conversion and reactor capacity were investigated.The continuous hydrolysis system was also analyzed by high performance size exclusion chromatography(HPSEC) and a mathematical model was established to describe the relationship between reaction conversion and modified space-time.The characteristic of hydrolysis process in a CSTR/UF was compared with BSTR.The results show that taking advantage of the integrated reaction-separation technique,the productivity or utilization efficiency of enzyme can be increased greatly,and the product is purified simultaneously.Therefore,enzymatic membrane reactor provided a more efficient way to prepare active peptides.