| HPLC测定二氟泼尼酯眼用乳剂的含量 |
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| 引用本文： | 王金虎,郭文建. HPLC测定二氟泼尼酯眼用乳剂的含量[J]. 山东食品科技, 2012, 0(11): 428-430 |
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| 作者姓名： | 王金虎 郭文建 |
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| 作者单位： | [1]山东省医药工业研究所,山东济南250101 [2]山东省化药重点室验室,山东济南250101 |
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| 摘 要： | 建立高效液相色谱法测定二氟泼尼酯眼用乳剂中二氟泼尼酯的含量。方法采用SHIMADZU VP-ODS C18（150mm×4．6mm，5μm）色谱柱，流动相为甲醇-水（52：48），检测波长240nm，流速1．0mL／min，柱温35℃。结果二氟泼尼酯在2．034-20．34μg/mL浓度范围内与峰面积有良好的线性关系，平均回收率98．80％。结论此方法结果简便，准确，重复性好，可以用于二氟泼尼酯眼用乳剂的质量控制。
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| 关 键 词： | 高效液相色谱法 二氟泼尼酯 含量测定 |
Determination of the Difluprednate Ophthalmic Emulsion by I-IPLC |
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| WANG Jin-hu,GUO Wen-jian. Determination of the Difluprednate Ophthalmic Emulsion by I-IPLC[J]. , 2012, 0(11): 428-430 |
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| Authors: | WANG Jin-hu GUO Wen-jian |
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| Affiliation: | 1. Shandong Institute of Pharmaceutical Industry, Jinan 250101 China, 2. Shandong Provincial Key Laboratory of Chemical Drug, Jinan 250101 China) |
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| Abstract: | Objective To establish an HPLC method for the determination of the difluprednate in difluprednate ophthalmic emulsion. Methods SHIMADZU VP-ODS C18 column （150 mm×4.6 mm, 5 μm） was adopted, the methanol-water （52：48） was used as mobile phase, the detection wavelength was 240 nm, the flow rate was 1.0 mL/ min, and the column temperature was 40 ℃. Results The linear range of difluprednate was 2.034- 20.34 μg/mL, the average recovery rate was 98.80%. Conclusion The method was simple, accurate and reproducible, can be used for quality control of difluprednate ophthalmic emulsion. |
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| Keywords: | HPLC difluprednate assay |
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