液相色谱-串联质谱法同时测定人血浆中罗通定、舒必利浓度

采用HPLC-MS/MS法同时测定人血浆中罗通定、舒必利浓度。以维拉帕米为内标，血浆经NaOH溶液碱化后，用二氯甲烷液-液萃取，以液相色谱分离、电喷雾离子化串联质谱进行检测，采用多反应监测模式同时测定罗通定（m/z 356.4/340.4）和舒必利（m/z 342.3/214.1）浓度。罗通定及舒必利标准曲线的线性范围分别是0.781 3～400 μg?L^-1 （r=0.999 3）和 3.125～1 600 μg?L^-1（r=0.999 3）；方法学回收率分别是95.3 %~100.8 % 和98.6%~102.4%；日内精密度分别为2.78%～6.79%和1.56%～2.63%；日间精密度分别为0.32%～2.22%和0.80%～1.41%。该方法能简便、快速、准确地测定人血浆中罗通定和舒必利浓度，可用于罗通定和舒必利的药动学及其他相关研究。

Simultaneous Quantitative Determination of Rotundine and Sulpiride in Human Plasma with Liquid Chromatography-Tandem Mass Spectrometry

The method for simultaneous quantitative determination of Rotundine and Sulpiride in human plasma was established by HPLC-MS/MS. Verapamil was chosen as internal standard. Sample of plasma was alkalified by sodium hydroxide, then extracted with dichloromethane. Rotundine （m/z 356.4/340.4） and Sulpiride（m/z 342.3/214.1）were detected by LC-MS/MS with multiple reaction monitoring mode. The standard curve of Rotundine and Sulpiride are linear in the concentration range of 0.781 3-400 μg?L^-1 (r=0.999 3) and 3.125-1 600 μg?L^-1 (r=0.999 3)， respectively. The method recovery of Rotundine and Sulpiride are 95.3 %-100.8 % and 98.6% 102.4%. The intra day RSD are 2.78%-6.79%and 1.56%-2.63%. The inter-day RSD are 0.32%-2.22% and 0.80%-1.41%. This method is simple, rapid, sensitive and accurate for determination of Rotundine and Sulpiride simultaneously. It can be used in pharmacokinetic for studying Rotundine and Sulpiride.