高选择性脂肪酶的筛选及其拆分布洛芬

为了实现脂肪酶在有机相中对外消旋布洛芬的高效拆分，筛选得到了一株具有高立体选择性的脂肪酶产生菌-扩展青霉TS414(Penicillium expansum TS414). 实验探讨了水分、温度、有机溶剂、酶浓度、醇结构和醇浓度对酶促拆分反应的影响，确立了酯化反应的最佳反应体系：布洛芬6.46 mmol/L，脂肪酶53.3 mg/mL，正丙醇40 mmol/L，异辛烷15 mL，水0.5%(j)，60℃条件下反应50 h. 在此条件下，拆分反应的转化率和对映体选择率分别为42%和429.63. 结果表明，Penicillium expansum TS414脂肪酶是一种较为理想的用于外消旋布洛芬拆分的工具酶，在布洛芬手性拆分方面具有广阔的应用前景.

Screening of a Lipase with High Enantioselectivity and Its Application in the Resolution of Racemic Ibuprofen

In order to resolute racemic ibuprofen enantioselectively in organic solvent, a strain of Penicillium expansum TS414 was screened, which could secrete a lipase with perfect enantioselectivity. The main variables affecting the resolution reaction by the lipase were optimized, including water content, temperature, organic solvent, enzyme concentration, structure and concentration of alcohol. Under the optimum conditions of 6.46 mmol/L ibuprofen, 53.3 g/L lipase, 40 mmol/L 1-propanol, 15 mL isooctane, 0.5%(j) water, and 60℃ for 50 h, the total conversion rate and the enantiomeric ratio could reach 42% and 429.63, respectively. The results showed that Penicillium expansum TS414 lipase could be used as a perfect tool enzyme with high enantioselectivity and had a great potential for commercial applications in the resolution of racemic ibuprofen.