| 纤维蛋白原制剂灭活病毒工艺的初步研究 |
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| 引用本文： | 王小良 韩剑秋. 纤维蛋白原制剂灭活病毒工艺的初步研究[J]. 中国生物制品学杂志, 1993, 6(3): 118-121 |
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| 作者姓名： | 王小良 韩剑秋 |
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| 作者单位： | 卫生部上海生物制品研究所，卫生部上海生物制品研究所，卫生部上海生物制品研究所，卫生部上海生物制品研究所，卫生部上海生物制品研究所，卫生部上海生物制品研究所 上海 200052，上海 200052，上海 200052，上海 200052，上海 200052，上海 200052 |
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| 摘 要： | 在纤维蛋白原(简称纤原)常规生产工艺中增加“有机溶剂/表面活性剂(S/D)”灭活病毒步骤·TNBP及Tween-80浓度分别为0.3％及1％,在24℃处理6小时,能有效灭活标志病毒VSV(7.31g_(10))及Sindbis V(6.831g_(10))。残余的TNBP及Tween-80可通过两次8％乙醇沉淀去除。病毒灭活后纤原的可凝固蛋白纯度由原来的70.9±8.5％提高至85.5±6.0％,回收率可保持在64.7±3.8％。PAGE电泳显示来出现新的蛋白区带。
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| 关 键 词： | 纤维蛋白原 灭活病毒 S/D处理 |
Preliminary Study on Virus Inactivation Procedure for Preparation of Fibrinogen |
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| Wang Xiaoliang. Preliminary Study on Virus Inactivation Procedure for Preparation of Fibrinogen[J]. Chinese Journal of Bilogicals, 1993, 6(3): 118-121 |
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| Authors: | Wang Xiaoliang |
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| Affiliation: | Wang Xiaoliang Shanghai Institute of Biological Products,Shanghai |
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| Abstract: | Conventional technology of preparing fibrinogen was improved by developing a procedure of virus inactivation by S/D. Marker viruses, VSV and Sindbis, could be effectively inactivated by 0.3% TNBP and 1% Tween-80 at 24℃ for 6 hours. After inactivation, the titers of two viruses were 7.31g10 and 6. 831g10, respectively. The residual TNBP and Tween-80 could be eliminated by twice precipitation with 8% ethanol. After virus inactivation, the purity of coagulable protein of fibrinogen was improved from 70.9±8. 5% to 85. 5±6. 0%, and the recovery rate was maintained at 64.7±3.8%. Detecting by PAGE, no new protein band was found. |
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| Keywords: | Fibrinogen Virus inactivation S/D treatment |
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