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Development of an SH-SAW sensor for the detection of DNA hybridization |
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| Authors: | Youngjune Jinho Jooheon Yukeun Eugene Yongrae |
| Affiliation: | aSchool of Mechanical Engineering, Kyungpook National University, Sankyukdong 1370, Bukgu, Daegu 702-701, Republic of Korea bDepartment of Sensor Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea cMEMS Lab., Materials and Devices Research Center, Samsung Advanced Institute of Technology, Suwon 449-712, Republic of Korea |
| Abstract: | We have developed SH (shear horizontal) surface acoustic wave (SAW) sensors for the detection of DNA (deoxyribonucleic acid) hybridization on the gold-coated delay line of transverse SAW devices. DNA hybridization experiments were performed with 15-mer oligonucleotides (probe and complementary target DNA). The sensor consists of twin SAW delay line oscillators (sensing channel and reference channel) operating at 100 MHz fabricated on 36° rotated Y-cut X-propagation LiTaO3 piezoelectric single crystals. The relative change in the frequency of the two oscillators was monitored to detect hybridization between the target DNA and probe DNA immobilized on the delay line of the SAW sensor. The measurement results showed a good response of the sensor to the mass loading effects of the DNA hybridization with a sensitivity level up to 1.55 ng/ml/Hz. |
| Keywords: | SH-SAW Oscillator Oligonucleotide DNA immobilization DNA hybridization |
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