| 高产胞外多糖嗜热链球菌的诱变育种研究 |
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| 引用本文： | 黄云鹏,姜淑娟,钱方,牟光庆.高产胞外多糖嗜热链球菌的诱变育种研究[J].食品工业,2012(6):43-45. |
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| 作者姓名： | 黄云鹏 姜淑娟 钱方 牟光庆 |
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| 作者单位： | 大连工业大学食品学院 |
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| 基金项目： | 辽宁省教育厅创新团队项目,项目编号2009T013 |
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| 摘 要： | 以一株从西藏雪莲中分离的产胞外多糖的嗜热链球菌(多糖产量343 mg/L)为出发菌株,采用紫外线和亚硝酸相结合的方法对其进行诱变研究。用薄层色谱和苯酚硫酸法进行筛选,获得一株产糖能力较高的突变菌株(多糖产量459 mg/L),其多糖产量较出发菌株提高了33.8%,遗传稳定性良好。
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| 关 键 词： | 嗜热链球菌 诱变 胞外多糖 遗传稳定性 |
Mutagenesis Breeding Research of High EPS-producing Streptococcus thermophilus |
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| Huang Yun-peng,Jiang Shu-juan,Qian Fang,Mu Guang-qing.Mutagenesis Breeding Research of High EPS-producing Streptococcus thermophilus[J].The Food Industry,2012(6):43-45. |
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| Authors: | Huang Yun-peng Jiang Shu-juan Qian Fang Mu Guang-qing |
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| Affiliation: | School of Food Engineering,Dalian Polytechnic University(Dalian 116034) |
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| Abstract: | A high EPS-producing streptococcus thermophilus strain was isolated from Tibetan Saussurea,and was mutated by UV and HNO2 environment.Thin Layer Chromatography and Phenol Sulfuric Acid Method were adopted for screening the mutant strains,a mutant strain was obtained with a EPS yield of 459 mg/L,which was 33.8% higher than the original strain.Moreover,its high EPS-producing ability remained stable. |
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| Keywords: | Streptococcus thermophilus mutagenesis exopoIysaccharide(EPS) genetic stability |
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