Polysaccharide‐based artificial extracellular matrix: Preparation and characterization of three‐dimensional,macroporous chitosan,and heparin composite scaffold |
| |
| Authors: | Shu‐Huei Yu Yu‐Bey Wu Fwu‐Long Mi Shin‐Shing Shyu |
| |
| Affiliation: | 1. Department of Polymer Materials, Vanung University, Chung‐Li, Taiwan, Republic of China;2. Department of Chemical and Material Engineering, National Central University, Chung‐Li, Taiwan, Republic of China;3. Department of Biotechnology, Chung‐Li, Tao‐Yuan, Taiwan 320, Republic of China;4. Center of Polymer Material Research and Department of Cosmetic Science, Vanung University, Chung‐Li, Taiwan |
| |
| Abstract: | Scaffold‐guided tissue engineering based on synthetic and natural occurring polymers has gained many interests in recent year. In this study, the development of a chitosan‐heparin artificial extracellular matrix (AECM) is reported. Three‐dimensional, macroporous composite AECMs composed of heparin (Hep) and chitosan (Chito) were prepared by an interpolyelectrolyte complex/lyophilization method. The Chito‐Hep composite AECMs were, respectively, crosslinked with glutaraldehyde, as well as cocrosslinked with N,N‐(3‐dimethylaminopropyl)‐N′‐ethyl carbodiimide (EDC/NHS) and N‐hydroxysuccinimide (NHS). The crosslinking reactions were examined by FT‐IR analysis. In physiological buffer solution (PBS), the EDC/NHS‐crosslinked Chito‐Hep composite AECM showed a relative lower water retention ratio than its glutaraldehyde‐crosslinked counterparts. The EDC/NHS‐crosslinked Chito‐Hep composite AECMs showed excellent biocompatibility, according to the results of the in vitro cytotoxic test. This result suggested that the EDC/NHS‐crosslinked Chito‐Hep composite AECMs might be a potential biomaterial for scaffold‐guided tissue engineering applications. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008 |
| |
| Keywords: | chitosan heparin artificial extracellular matrix glutaraldehyde scaffold |
|
|
