Biosynthesis of unsaturated fatty acids in the diatomPhaeodactylum tricornutum |
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Authors: | Victor J Moreno Julia E A de Moreno Rodolfo R Brenner |
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Affiliation: | (1) Cátedra de Química Biológica, Dpto. de Biología, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina;(2) I.N.I.D.E.P., Argentina;(3) Cátedra de Bioquímica, Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina;(4) Present address: Instituto Nacional de Investigación y Desarrollo Pesquero, Casilla de Correo 175, 7600 Mar del Plata, Argentina |
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Abstract: | The biosynthesis of fatty acids in the diatomPhaeodactylum tricornutum was studied. The diatom was incubated with sodium 114C] acetate and the acids 1-14C] palmitic, 1-14C] stearic, 1-14C] linoleic and 1-14C] α-linolenic. The distribution of radioactivity in the products was determined by gas liquid radiochromatography. The diatom
synthesized “de novo” not only saturated and monounsaturated fatty acids, but also linoleic, α-linolenic and other fatty acids
including the highly polyunsaturated 20∶5ω3 and 22∶6ω3. When labeled acetate, stearic, α-linolenic or even linoleic acid were
incubated with the diatom, the polyunsaturated C20 fatty acids synthesized belonged predominantly to the ω 3 family. The existence of Δ9, Δ6, Δ5, Δ4, ω6 and possibly ω3 desaturases
inP. tricornutum is suggested.
Member of the Carrera del Investigador Científico of the Comisión de Investigaciones Científicas de la Provincia de Buenos
Aires.
Member of the Carrera del Investigador Cientifico of the Consejo Nacional de Investigaciones Cientificas y Técnicas. |
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