| 类金属硫蛋白产生菌的诱变育种 |
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| 引用本文： | 成玉梁,姚卫蓉,钱和,杨梅琳.类金属硫蛋白产生菌的诱变育种[J].食品研究与开发,2006,27(2):44-47,50. |
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| 作者姓名： | 成玉梁 姚卫蓉 钱和 杨梅琳 |
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| 作者单位： | 江南大学食品学院,江苏,无锡,214036 |
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| 摘 要： | 本研究选取酿酒酵母Cu-3作为产类金属硫蛋白的出发菌株，采用物理（UV诱变）以及化学诱变（NTG诱变）方法进行复合交替诱变，再进行类金属硫蛋白诱导实验，得到突变菌株N-1，其类金属硫蛋白Cu—MT产量有了十分明显的提高，并且具有很好的遗传稳定性。
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| 关 键 词： | 酿酒酵母 类金属硫蛋白 |
| 收稿时间： | 2005-11-15 |
| 修稿时间： | 2005-11-15 |
THE STUDY OF MUTATION AND BREEDING OF THE STRAINS PRODUCING MT-LIKE |
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| CHENG Yu-liang,YAO Wei-rong,QIAN He,YANG Mei-lin.THE STUDY OF MUTATION AND BREEDING OF THE STRAINS PRODUCING MT-LIKE[J].Food Research and Developent,2006,27(2):44-47,50. |
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| Authors: | CHENG Yu-liang YAO Wei-rong QIAN He YANG Mei-lin |
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| Affiliation: | Food Department,Southern Yangtze University, Wuxi 214036,China |
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| Abstract: | In this article, Saccharomyces cerevisiae Cu-3 which had a high productivity of MT-like was selected as the starting strain for stepwise mutation by UV irradiation and N-methyl-N'-nitro-soguanidine(NTG) treatment. And a Cu-MT high-producing mutant N-1 was finally obtained, with a perfect transmissibility. |
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| Keywords: | UV NTG Cu-MT |
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