| 低剂量X射线照射对J774A.1细胞IL-12和 p38 MAPK表达的影响 |
| |
| 引用本文： | 刘晓冬,刘树铮,马淑梅,刘扬.低剂量X射线照射对J774A.1细胞IL-12和 p38 MAPK表达的影响[J].辐射研究与辐射工艺学报,2002,20(4):271-274. |
| |
| 作者姓名： | 刘晓冬 刘树铮 马淑梅 刘扬 |
| |
| 作者单位： | 吉林大学白求恩医学部卫生部放射生物学重点实验室,长春,130021 |
| |
| 基金项目： | 国家自然科学基金，39570188， |
| |
| 摘 要： | 通过检测低剂量辐射(LDR)对J774A.1细胞株中白细胞介素12(IL-12)及p38丝裂原活化蛋白激酶(p38 MAPK)的影响,以研究LDR辐射免疫效应的机制.分别采用流式细胞术和ELISA检测了p38 MAPK、IL-12 蛋白水平的变化.结果表明,75mGy X射线照射后,J774A.1细胞分泌IL-12量在照射后2、4、8h升高(p<0.05),随后即开始下降至恢复假照射水平;而p38 MAPK也于照射后2h出现升高,两者在发生时间上呈现一致性.结果显示LDR引起IL-12表达增加,而p38 MAPK传导通路可能在其中发挥作用,为低剂量辐射增强细胞免疫功能提供了实验依据.
|
| 关 键 词： | LDR 辐射兴奋效应 |
| 修稿时间： | 2002年1月11日 |
INFLUENCE OF LOW DOSE X RAYS IRRADIATION ON IL-12 AND p38 MAPK EXPRESSION IN J774A.1 CELL LINE |
| |
| LIU Xiaodong,LIU Shuzheng,MA Shumei,LIU Yang.INFLUENCE OF LOW DOSE X RAYS IRRADIATION ON IL-12 AND p38 MAPK EXPRESSION IN J774A.1 CELL LINE[J].Journal of Radiation Research and Radiation Processing,2002,20(4):271-274. |
| |
| Authors: | LIU Xiaodong LIU Shuzheng MA Shumei LIU Yang |
| |
| Abstract: | To observe the effects of LDR on the p38 MAPK expression and the secretion of IL-12 in J774A.1 cell line, we used ELISA and flow cytometry to examine the changes in these two specialities. Results showed that IL-12 p70 in J774A.1 increased 2-8h after 75mGy X irradiation, and consistently, p38 MAPK expression increased at the 2nd h after exposure. Both of these increases showed statistic significance. Conclusions were drawn that LDR might stimulate IL-12 expression ,and p38 MAPKmight play some role in the activation of IL-12, which would provide theoretic basis for the hormesis effect of LDR. |
| |
| Keywords: | IL-12 p38 MAPK |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
