食品检测即用型液体培养基产品的评价研究

目的对即用型缓冲蛋白胨水、3%氯化钠碱性蛋白胨水、月桂基硫酸盐胰蛋白胨肉汤培养基进行质量评价。方法使用即用型培养基和新鲜配制的培养基对已完成生化确认的10株沙门氏菌、5株阪崎肠杆菌、15株副溶血性弧菌、15株肠杆菌科细菌共45株目标菌及5株干扰菌进行检测,确定菌株的灵敏度和特异性;平行培养0、6和24 h后对比即用型培养基增菌效果与新鲜配制的培养基是否存在差异。结果使用即用型培养基与新鲜配制的培养基对全部目标菌与干扰菌的6 h、24 h细菌生长的检验结果未见显著差异(全部单因素方法分析结果均为P0.05)。结论被测试目标菌及干扰菌在即用型培养基中的生长结果可满足GB 4789.28-2013对非选择性及选择性增菌培养基的质量要求。

Research and evaluation on the ready-to-use culture medium in food detection

Objective To conduct quality assessment of ready-to-use buffered peptone water, 3% sodium chloride alkaline peptone water and lauryl sulfate tryptone (LST) broth. Method Using ready-to-use culture medium and freshly prepared culture medium, the sensibility and specificity of 45 strains of target bacteria which had completed the biochemical confirmation, including 10 strains of Salmonella, 5 strains of Enterobacter sakazakii, 15 strains of Vibrio parahaemolyticus and 15 strains of enterobacteriaceae bacteria, and 5 strains of interfering bacteria were detected and confirmed. After parallel cultivation for 0, 6 and 24 h, ready-to-use culture medium and freshly prepared culture medium were contrasted to find whether there was a significant difference. Results There were no significant difference between ready-to-use culture medium and freshly prepared culture medium through cultivation of target bacteria and interfering bacteria for 6 h and 24 h, and P values were all greater than 0.05. Conclusion In ready-to-use culture medium, the growth rates of target bacteria and interfering bacteria can satisfy the requirements of GB 4789.28-2013 for nonselective and selective enrichment medium.