Benign reflex myoclonic epilepsy in infants

A technique for NADH-diaphorase and glucose-6-phosphatase activity visualization at the light microscope level has been essentially modified by the use of a short pre-fixation of cryostat sections in a gluteraldehyde-containing fixative followed by a prolonged (18-20 h) incubation in the chilled (4-6 degrees C) standard media. Besides, for revealing NADH-diaphorase Triton X-100 is recommended to add to the incubation medium. The offered technical modifications secure a high staining intensity and specificity of both histochemical reactions tested without any substantial sophistication of the procedure.