高产花生四烯酸高山被孢霉的诱变育种研究

通过菌种选育获得具有优良产脂性能的菌株是实现微生物油脂工业化生产的重要前提。利用实验室前期构建的产油丝状真菌快速筛选技术,以前期筛选获得的一株性状优良的高山被孢霉TSM-3为出发菌株,通过常压室温等离子体(ARTP)诱变方法得到能够稳定遗传的高产突变菌株TSM-3-1,其油脂产量和花生四烯酸(AA)产量分别达到5.07 g/L和1.55 g/L,与出发菌株相比分别提高了47.81%和84.52%。将产油丝状真菌快速筛选技术与诱变育种相结合,提高了目标菌株筛选效率并强化了野生型菌株油脂合成能力,为产油丝状真菌菌种选育提供了新颖的研究思路及方法。

Mutation breeding of high arachidonic acid-producing strain in Mortierella alpina

Obtaining the strains with good properties through breeding technologies is the precondition of achieving industrial production of microbial oil. Mortierella alpina TSM-3 was selected as the original strain, which was screened through a quick and efficient screening strategy for oleaginous filamentous fungi established by laboratory before. By the method of atmospheric and room temperature plasma (ARTP) mutagenesis, the oil and arachidonic acid(AA) yields reached 5.07 g/L and 1.55 g/L in the stable genetic mutant strain M. alpina TSM-3-1, which increased by 47.81% and 84.52% compared with the original strain. By means of combining the quick and efficient screening strategy with ARTP mutation breeding, the screening efficiency and oil producing capacity of wild strain were well enhanced, which provided a novel research strategy in the study of oleaginous filamentous fungi.