| 利用流式细胞仪筛选紫外诱变高含油小球藻 |
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| 引用本文： | 李青,吴洪,蔡忠贞,等.利用流式细胞仪筛选紫外诱变高含油小球藻[J].中国油脂,2018,43(5). |
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| 作者姓名： | 李青 吴洪 蔡忠贞 等 |
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| 作者单位： | 新奥科技发展有限公司煤基低碳能源国家重点实验室 |
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| 摘 要： | 为筛选得到高含油的小球藻藻株,对若夫小球藻(Chlorella zofingensis)进行紫外诱变,尼罗红染色后再利用流式细胞仪对高含油藻株进行筛选。结果表明:紫外诱变40 min后,经流式细胞仪筛选,分选出的90%的突变藻株总脂含量都高于野生对照株。最终筛选到的藻株S16经培养总脂产量达到2.4 g/L,比野生对照株提高了50%。
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| 关 键 词： | 小球藻 紫外诱变 流式细胞仪分选 高含油 |
Screening of UV-mutated Chlorella zofingensis with high lipid content using flow cytometry |
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| LI Qing,WU Hong,CAI Zhongzhen,?etc.Screening of UV-mutated Chlorella zofingensis with high lipid content using flow cytometry[J].China Oils and Fats,2018,43(5). |
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| Authors: | LI Qing WU Hong CAI Zhongzhen ?etc |
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| Abstract: | In order to screen strain with high lipid content from Chlorella zofingensis, firstly Chlorella zofingensis was mutated by ultraviolet ray(UV), and then the cells of mutated Chlorella zofingensis were screened by flow cytometry after Nile red staining. The results showed that after being mutated by UV for 40 min and screened by flow cytometry, 90% of the mutated strains contained more lipid than wild control strain. The total lipid yield of the screened strain S16 reached 2.4 g/L, increasing by 50% compared with wild control strain. |
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| Keywords: | Chlorella zofingensis UV-mutated mutation flow cytometry screening high lipid content |
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