磷脂酰丝氨酸的酶法制备与分离研究进展

磷脂酰丝氨酸(PS)的制备有溶剂提取法、化学合成法和酶转化法。采用溶剂提取法从动物组织提取PS由于"疯牛病"原因而受质疑;化学合成法成本较高,工艺复杂,且纯度和收率有待提高;酶转化法具有反应条件温和、反应易控、高效简单的优势,越来越受到关注。主要对磷脂酰丝氨酸的酶法制备以及分离纯化方法进行了综述,对其生产制备过程提出了合理的建议和策略。

Advance in enzymatic preparation and separation of phosphatidylserine

Phosphatidylserine(PS) can be prepared by solvent extraction, chemical synthesis and enzymatic synthesis. PS extracted from animal tissues by solvent extraction is questioned because of bovine spongiform encephalopathy.Chemical synthesis has the weakness of high cost and complex process, also the purity and yield need to be improved. Enzymatic synthesis has the advantages of mild conditions, easy to control and high efficiency, and it attracts a lot of attention. The preparation of PS by enzymatic synthesis and the separation and purification methods were reviewed, and some reasonable suggestions and strategies were put forward.