| 高效液相色谱法测定烟草中的果糖、葡萄糖和蔗糖 |
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| 引用本文： | 黄翼飞.高效液相色谱法测定烟草中的果糖、葡萄糖和蔗糖[J].烟草科技,2010(1). |
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| 作者姓名： | 黄翼飞 |
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| 作者单位： | 广东中烟工业有限责任公司技术中心,广州市中山七路333号,510145 |
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| 摘 要： | 以72%(体积比)乙腈/28%水预混合溶液作流动相,High-Performance Carbohydrate卡套柱作色谱柱,等梯度洗脱,采用带示差折光检测器的高效液相色谱仪测定了烟草中的果糖、葡萄糖和蔗糖含量。结果表明:方法的日内精密度和日间精密度的范围分别为0.46%~1.54%和0.96%~4.74%,回收率97.0%~102.3%,果糖、葡萄糖和蔗糖的检出限(LOD)分别为95,107和105μg/mL(S/N=3∶1),可以满足烟草中果糖、葡萄糖和蔗糖含量的日常检测要求。
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| 关 键 词： | 高效液相色谱 示差折光 果糖 葡萄糖 蔗糖 烟草 |
Determination of Fructose,Glucose and Sucrose in Tobacco with High Performance Liquid Chromatography |
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| HUANG YI-FEI.Determination of Fructose,Glucose and Sucrose in Tobacco with High Performance Liquid Chromatography[J].Tobacco Science & Technology,2010(1). |
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| Authors: | HUANG YI-FEI |
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| Affiliation: | HUANG YI-FEITechnology Center,China Tobacco Guangdong Industry Co.,Ltd.,Guangzhou 510145,China |
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| Abstract: | The fructose,glucose and sucrose in tobacco were determined by high performance liquid chromatography with a High-Performance Carbohydrate cartridge column and a differential refractive index detector at isocratic mode using a premixed solution of 72% acetonitrile-28% water(v/v) as an eluent.The results showed that intra-day and inter-day precisions were in the range of 0.46%-1.54% and 0.96%-4.74%,respectively.The recovery was between 97.0%-102.3%.The limits of detection of fructose,glucose and sucrose were... |
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| Keywords: | High performance liquid chromatography Differential refractive index Fructose Glucose Sucrose Tobacco |
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