辐射聚合覆盖离子型高分子的纸固定化绿色木霉细胞

通过辐射聚合,在纸表面覆盖阳离子——疏水性共聚物polo(DEAEMA-ATMPT)和阴离子——疏水性共聚物poly(AA-ATMPT)作为固定化绿色木霉细胞的载体。试验结果表明:用覆盖poly(DEAEMA-ATMPT)的载体固定化细胞比较牢固,未因振荡而解吸;固定化细胞的FPA(滤纸活性)均高于未固定化的游离细胞,其中用覆盖25％:75％ poly(DEAEMA-ATMPT)的载体固定化细胞的FPA比游离细胞增加了20％;固定化细胞的重量随共聚物中阳离子组分的增加而增加。用覆盖poly(AA-ATMPT)的载体固定化绿色木霉也有较好的效果。但固定化细胞的重量明显低于覆盖poly(DEAEMA-ATMPT)的载体固定化细胞的重量,且它随共聚物中阴离子组分的增加而下降。阳离子——疏水性共聚物能提高固定化绿色水霉的效果;用离子型共聚材料固定化细胞静电引力起了主要的作用。

IMMOBILIZATION OF TRICHODERMA REESEI (QM 9414) CELLS WITH PAPER COVERED WITH IONIC COPOLYMER BY RADIATION POLYMERIZATION

Cationic-hydrophobic copolymer and anionic-hydrophobic copolymer was covered onto surface of paper by radiation polymerization. The paper covered wi- th ionic copolymer was used as carrier of immobilizing Trichoderma reesei cells. Results showed that the cells were immobilized firmly on the carriers and not dislocated from the carriers by shaking. All of FPA of the cells immobilized with. the carriers covered with cationic copolymer were higher than that of unimmobilized free cells. The carriers covered with anionic copolymer showed good effect on immobilization of the cells. The weight of immobilized cells increase as increasing the component of DEAEMA in po- ly (DEAEMA-ATMPT) or decreasing the component of AA in poly (AA-ATMPT). It also increase with the increese of water absorption in poly (DEAEMA-ATMPT) or decrease of water absorption in poly (AA-ATMPT). It shows tha static interatcion play an important role in the immon?ization of cells with ionic copolymer materials.