| 毕赤酵母木聚糖酶的活力测定条件研究 |
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| 引用本文： | 万红贵,李喜连,贾伟. 毕赤酵母木聚糖酶的活力测定条件研究[J]. 食品与发酵工业, 2007, 33(9): 150-153 |
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| 作者姓名： | 万红贵 李喜连 贾伟 |
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| 作者单位： | 南京工业大学制药与生命科学学院,江苏南京,210009 |
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| 摘 要： | 为探讨毕赤酵母木聚糖酶的催化特性以及更好地发挥其催化功能,从pH、温度、底物浓度、反应时间、DNS用量、DNS显色时间等几个方面研究了木聚糖酶活性测定的最佳条件。研究结果表明,木聚糖酶酶活测定的适宜条件为:1%的木聚糖用0.05mol/L pH6.0的柠檬酸缓冲液配制;测定温度70℃,且该酶的热稳定性较好.在55～60℃下放置2h能保持68%以上的酶活;酶促反应时间10min;DNS用量3mL;DNS显色时间5min。
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| 关 键 词： | 毕赤酵母 木聚糖酶 酶活力 测定条件 |
| 收稿时间： | 2007-04-25 |
| 修稿时间： | 2007-04-25 |
Studies on Method of Measuring the Activity of Xylanase from Pichia |
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| Wan Honggui,Li Xilian,Jia Wei. Studies on Method of Measuring the Activity of Xylanase from Pichia[J]. Food and Fermentation Industries, 2007, 33(9): 150-153 |
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| Authors: | Wan Honggui Li Xilian Jia Wei |
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| Abstract: | To find out the catalysis character of xylanase in Pichia and exhibit its maximum catalysis rate, pH value,reaction temperature,substrate concentration,reaction time,the quantity of DNS,coloration time etc.were researched to determined the optimum conditions.The results showed that the optimum con- ditions for determination high xylanase activity were 1% xylan resolution,pH6.0,0.05mol/L citric acid buffer,reaction temperature of 70℃and the thermal stability of xylanase was preferable between 55～60℃to keep relative xylanase activity above 68%,reaction time 10 min,DNS 3mL,coloration time 5rain for xyla- nase activity. |
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| Keywords: | Pichia xylanase enzyme activity measurement condition |
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