| 黄粉虫中提取纯化超氧化歧化酶的工艺研究 |
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| 引用本文： | 吴蕾,陈庆森,刘晋生.黄粉虫中提取纯化超氧化歧化酶的工艺研究[J].食品科学,2007,28(9):281-283. |
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| 作者姓名： | 吴蕾 陈庆森 刘晋生 |
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| 作者单位： | 天津市食品生物技术重点实验室 天津商业大学生物工程系； 天津市食品生物技术重点实验室； 天津商业大学生物工程系； 天津大学化工学院生物工程系； 天津300134； 天津300072； |
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| 基金项目： | 天津市科技攻关计划重点专项项目（06YFGZNC04200） |
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| 摘 要： | 本研究以黄粉虫粉为原料,经盐析沉淀,DEAE-Sepharose FF阴离子交换柱层析和Sephadex G200凝胶过滤层析等纯化步骤,获得了其中的一种超氧化物歧化酶(SOD)。酶的纯化倍数为78.3倍。SDS-PAGE电泳显示为均一的蛋白谱带。经鉴定该酶为Cu.Zn-SOD。本研究为从黄粉虫中提取SOD提供了一种较好的工艺。
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| 关 键 词： | 黄粉虫 超氧化歧化酶 提取 纯化 柱层析 |
| 文章编号： | 1002-6630（2007）09-0281-03 |
| 修稿时间： | 2007-07-19 |
Study on Technology of Extraction and Purification Superoxide Dismutase from Yellow Mealworm |
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| WU Lei,CHEN Qing-sen,LIU Jin-sheng.Study on Technology of Extraction and Purification Superoxide Dismutase from Yellow Mealworm[J].Food Science,2007,28(9):281-283. |
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| Authors: | WU Lei CHEN Qing-sen LIU Jin-sheng |
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| Affiliation: | 1.Tianjin Key Laboratory of Food and Biotechnology,Department of Bioengineering,Tianjin University of Commerce,Tianjin 300134,China;2.School of Chemical-Engineering,Tianjin University,Tianjin 300072,China |
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| Abstract: | In this paper,a kind of superoxide dismutase(SOD)was obtained from yellow worm powder.Two-step(NH4)2SO4 precipitation were used as the main extraction method and DEAE-Sepharose FF ion-exchange and Sephadex G200 volume exclude chromatography were used as purification methods.The purification factor was 78.3.The SDS-PAGE showed that the SOD was a homogenous protein.This enzyme was proved as Cu.Zn-SOD.This study provides a suitable technology for obtaining SOD from the yellow mealworm. |
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| Keywords: | yellow mealworm SOD extraction purification chromatography |
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