| 亲和素-生物素系统的153Sm标记及其生物学分布动力学的研究 |
| |
| 引用本文： | 李贵平,朱承谟,江旭峰,冯国伟,张圣国. 亲和素-生物素系统的153Sm标记及其生物学分布动力学的研究[J]. 核技术, 1999, 22(11): 686-689 |
| |
| 作者姓名： | 李贵平 朱承谟 江旭峰 冯国伟 张圣国 |
| |
| 作者单位： | 上海第二医科大学附属瑞金医院,上海,200025 |
| |
| 摘 要： | 选用＾１５３Ｓｍ对生物素进行放射性标记，然后利用亲和素和链霉亲和素与生物素的高亲合力特性，再对亲和素和链霉亲和素进行＾１５３Ｓｍ的标记，观察到大鼠和小鼠体内的血清除率和生物学分布，并与＾１５３ＳｍＣｌ３和＾１５３Ｓｍ－ＤＴＰＡ的生物学分布进行比较。结果表明，＾１５３Ｓｍ标记的亲和素血素除迅速，肝肾放射性摄取高；＾１５３Ｓｍ标记的链霉亲和素血清除缓慢，肝、脾、肾等脏器和血液中滞留量高；而＾１５３Ｓｍ
|
| 关 键 词： | 亲和素-生物素系统 153Sm标记 生物分布 |
The biodistribution and kinetics of the 153Sm labelled avidin,streptavidin and biotin |
| |
| LI Guiping,ZHU Chengmo,JIANG Xufeng,FENG Guowei,ZHANG Shenguo. The biodistribution and kinetics of the 153Sm labelled avidin,streptavidin and biotin[J]. Nuclear Techniques, 1999, 22(11): 686-689 |
| |
| Authors: | LI Guiping ZHU Chengmo JIANG Xufeng FENG Guowei ZHANG Shenguo |
| |
| Abstract: | Due to the high affinity of biotin to Av or SA. We labelled a biotin derivative(DTPA-biotin) with ~153Sm and then bound this 153Sm labelled DTPA-biotin to Av orSA. The in vivo kinetics and biodistribution of 153~Sm labelled, Av, SA andDTPA-biotin were studied in the rat and mice. The results demonstrated that153Sm-Av cleared from the blood rapidly with high liver and renal uptake; ~153Sm-SAcleared from blood slowly with high retention in liver, spleen and kidney, whereas153Sm metabolize more fast, and excreted mainly through the kidney. Thereby, thebiodistribution difference of SA and Av mentioned above provided an expermentalbasis for the selection of different components of A-V system in pretagetingradioimmuno imaging and radioimmunotherapy. |
| |
| Keywords: | Avidin-biotin system ~(153)Sm labelling Biodistribution |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
