| 一株多重耐药副溶血性弧菌的耐药分子机制研究 |
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| 引用本文： | 江艳华,姚琳,李风铃,翟毓秀,王联珠. 一株多重耐药副溶血性弧菌的耐药分子机制研究[J]. 食品安全质量检测学报, 2014, 5(1): 77-82 |
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| 作者姓名： | 江艳华 姚琳 李风铃 翟毓秀 王联珠 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所 农业部水产品质量安全检测与评价重点实验室,中国水产科学研究院黄海水产研究所 农业部水产品质量安全检测与评价重点实验室,中国水产科学研究院黄海水产研究所 农业部水产品质量安全检测与评价重点实验室,中国水产科学研究院黄海水产研究所 农业部水产品质量安全检测与评价重点实验室,中国水产科学研究院黄海水产研究所 农业部水产品质量安全检测与评价重点实验室 |
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| 基金项目： | 黄海水产研究所级基本科研业务经费(20603022012010) |
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| 摘 要： | 目的从养殖半滑舌鳎肠道分离到一株副溶血性弧菌Vp1107,对该菌株进行耐药性及耐药分子机制研究。方法通过K-B法进行药敏试验,PCR扩增及测序法对耐药基因及整合子进行检测与分析。结果 Vp1107菌株耐受氨苄西林、阿莫西林、头孢唑啉、四环素、土霉素和氯霉素,对头孢呋辛钠、链霉素、卡那霉素、萘啶酸、环丙沙星中度敏感,其多重耐药系数为0.33。菌株携带blaTEM、strA、strB、tetB、tetM、catⅢ、intI1耐药相关基因,1类整合子不含基因盒,gyrA和parC基因的喹诺酮类耐药决定区未发生点突变。结论副溶血性弧菌Vp1107耐药程度较为严重,其耐药性主要由耐药基因编码的耐药性酶类和外排泵作用引起,提示应加强对副溶血性弧菌耐药性的监控及渔用药物的管理。
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| 关 键 词： | 副溶血性弧菌 多重耐药性 耐药基因 整合子 喹诺酮耐药决定区 |
| 收稿时间： | 2013-10-14 |
| 修稿时间： | 2013-11-17 |
Studies on molecular mechanism of a multi-drug resistant Vibrio parahaemolyticus strain |
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| JIANG Yan-Hu,YAO Lin,LI Feng-Ling,ZHAI Yu-Xiu and WANG Lian-Zhu. Studies on molecular mechanism of a multi-drug resistant Vibrio parahaemolyticus strain[J]. Journal of Food Safety & Quality, 2014, 5(1): 77-82 |
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| Authors: | JIANG Yan-Hu YAO Lin LI Feng-Ling ZHAI Yu-Xiu WANG Lian-Zhu |
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| Affiliation: | Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences and Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
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| Abstract: | |
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| Keywords: | Vibrio parahaemolyticus multi-drug resistance drug resistance genes integrons quinolone resistance-determining region |
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