Mixed monolayer-protected gold nanoclusters as selective peptide extraction agents for MALDI-MS analysis |
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Authors: | Vanderpuije Benjamin N Y Han Gang Rotello Vincent M Vachet Richard W |
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Affiliation: | Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA. |
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Abstract: | Cationic and anionic nanoparticles selectively target peptides with low and high isoelectric points, respectively. Additionally, their high surface area-to-volume ratios make these nanoparticles (approximately 2-nm core diameter) very efficient extraction and concentration agents. Upon extraction, the peptide-bound nanoparticles can be analyzed by MALDI-MS to provide highly sensitive detection of the targeted peptides. We demonstrate that MALDI-MS can detect peptide concentrations as low as 500 pM from 250-microL solutions using these nanoparticle scaffolds as extraction and concentration agents. |
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