ALA联合HPD光动力学治疗对S180腹水瘤细胞的影响的研究

目的 :通过细胞学水平研究确定ALA配合小剂量HPD激光光动力疗法促使肿细胞死亡及凋亡的光敏药用药剂量及与二者单独使用的差别方法 :以不同剂量的ALA(40 μg/ml、80 μg/ml、16 0 μg/ml)、HPD(2 .5 μg/ml、5 μg/ml、10 μg/ml)、二者联合与S180腹水瘤细胞一起培养 ,随后以 6 30nm半导体激光 2 0 0mW /cm2 ,照射肿瘤细胞 ,照射 2 0分钟 ,及与不用光敏剂单照激光、单用光敏剂不照光、不用光敏剂不照激光空白对照组比较。以流式细胞仪及倒置微镜观察促使肿瘤细胞凋亡或死亡的差别 ,寻找促使肿瘤细胞凋亡或死亡的最小合适剂量。结果 :ALA4 0 μg/ml配合HPD2 .5 μg/ml与S180腹水瘤细胞同时培养 ,6 30半导体激光 2 0 0mW /cm2 ,照射 2 0分钟组从形态学观察及流式细胞仪测定是能达到较佳的细胞凋亡的最小用光敏剂剂量。单纯ALA -PDT组中能达到小鼠S180腹水瘤细胞明显凋亡的最小剂量是ALA(80 μg/ml) ,单纯HPD -PDT组中能达到小鼠S180腹水瘤细胞明显凋亡最小剂量是HPD(5 μg/ml)。结论 :ALA4 0mg/kg配合HPD2 .5mg/kg ,6 30半导体激光2 0 0mW /cm2 ,照射 2 0分钟是能达到小鼠S180腹水瘤细胞明显凋亡的最小光敏剂剂量。

Research of ALA Combined with HpD-PDT Which Induced S180 Ascitic Tumor Cells Death or Apoptosis on Cytology

Objective:to ascertain the adequate dosage of ALA combined with HpD-PDT which induced tumor cell death or apoptosis on cytology.And to study the different effect of ALA-PDT and HPD-PDT used only.Methode:Rat ascitic tumor cells(S180) were randomly divided into several groups and incubated with ALA (20ug/ml、 40ug/ml 、160ng/ml)HPD(2.5ug/ml 、5ug/ml 、10ug/ml) and their combination dosages.630nm light (total output 2W)was delivered to tumor cells at a constant fluence rate:200mW/cm2and a constant irradiated tume period:20 minrtes.We set 3 groups (no photosensitizers or no irradiation or neither)to be the control groups.We used incersion microscopy to observe the morphological exchange of tumor cells and flow cytometry technology to detect the death or apoptosis of tumor cells during the exchange of tumor cells and flow cytometru technologh to detect the death or apoptosis of tumor cells durint the experiment.Results:After irradiated with 630nm light on 20 minutes S180 tumor cells incubated with the combination dosage of ALA 40ug/ml and HPD2.5ug/ml were induced highest rate of apoptosis.The rate of cells early apoptosis was 2.54%,while the late apoptosis was 95.105.Conclusion:The combination dosage of ALA 40ug/ml and HPD2.5ug/ml(25% of constant dosage)used in the PDT treatment was the most adequte dosage on cytology.