Detection of anti-HIV-1 IgG antibodies in whole saliva by GACELISA and Western blot assays |
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Authors: | MI Matee EF Lyamuya E Simon EC Mbena C Kagoma LP Samaranayake F Scheutz |
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Affiliation: | Department of Microbiology and Immunology, Muhimbili University College of Health Sciences, Dar es Salaam, Tanzania. |
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Abstract: | The present study, based on 158 HIV seropositives and 167 HIV seronegatives, demonstrates that saliva collected with the Omni-SAL device and tested with GACELISA (an IgG antibody capture ELISA) is an effective non-invasive alternative to serum for anti-HIV IgG antibody screening. The study also shows that a conventional serum Western blot kit can be used, with slight modifications, for confirmatory testing of saliva specimens. Collecting saliva with the Omni-SAL device had a very good acceptance rate among Tanzanian subjects, and although this diagnostic method is not yet known by the general public, 65% of the study participants preferred to give saliva instead of blood for HIV testing. |
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