| HPLC法检测牛初乳中lgG含量 |
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| 引用本文： | 熊勇华,许杨,魏华,曾荣华.HPLC法检测牛初乳中lgG含量[J].食品工业科技,2003(9). |
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| 作者姓名： | 熊勇华 许杨 魏华 曾荣华 |
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| 作者单位： | 中德联合研究院食品科学教育部国家重点实验室，中德联合研究院食品科学教育部国家重点实验室，中德联合研究院食品科学教育部国家重点实验室，中德联合研究院食品科学教育部国家重点实验室 南昌 330047，南昌 330047，南昌 330047，南昌 330047 |
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| 基金项目： | 国家计委高新技术产业化示范工程项目，江西省主要学科跨世纪学术和技术带头人培养计划资助项目 |
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| 摘 要： | 建立了牛初乳中IgG的高效液相色谱检测法,并测定了 初乳中IgG的经时变化过程。结果显示,IgG浓度在0.2～ 15mg/mL的范围内峰面积与浓度呈现良好的线性关系, 回收率实验平均大于98.5％。采用本法测定了六头幽门螺 杆菌免疫牛初乳中IgG及两头正常牛初乳中IgG的经时 变化过程。母牛分娩后24h内,初乳中免疫球蛋白IgG平 均含量在40～70mg/mL之间;3d后,初乳中免疫球蛋白 总量下降较快;至第7d,初乳中免疫球蛋白含量平均在 1～5mg/mL之间。
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| 关 键 词： | 牛初乳 IgG |
Determination of lgG in boving colostrum by HPLC |
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| Xiong Yonghua et al.Determination of lgG in boving colostrum by HPLC[J].Science and Technology of Food Industry,2003(9). |
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| Authors: | Xiong Yonghua |
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| Affiliation: | Xiong Yonghua et al |
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| Abstract: | ln this paper,the method of determining the concentration of IgG in bovine colostrum by HPLC was set up and the content of IgG in colostrum changing with time was detected. The result showed that there was a nice linear relationship between the peak area and the content of IgG in the range of 0.2~15mg/mL The average recover rate was 98.5% The change in the content of IgG with time in six immuned bovine colostrum and two normal bovine colostrum were determined using this method. The average content of IgG in colostrum was in 40 ~70mg/mL in the range of 24h after cow delivery, and decreased quickly after three days. On the seventh day, the content of IgG in colostrum was 1~5mg/mL. |
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| Keywords: | bovine colostrum IgG |
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