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| 仔猪复合微生态制剂的液体发酵工艺优化 | |
| 引用本文： | 王建涛,付桂明,刘成梅,万茵,李红歌,柴建新,陈建芳,胡明,白鲁杰. 仔猪复合微生态制剂的液体发酵工艺优化[J]. 粮食与饲料工业, 2012, 12(1): 42-45 |
| 作者姓名： | 王建涛 付桂明 刘成梅 万茵 李红歌 柴建新 陈建芳 胡明 白鲁杰 |
| 作者单位： | 1. 南昌大学食品科学与技术国家重点实验室,江西南昌 330047;南昌大学生命科学与食品工程学院,江西南昌330047;南昌大学中德食品工程中心,江西南昌 330047 2. 南昌大学生命科学与食品工程学院,江西南昌330047;南昌大学中德食品工程中心,江西南昌 330047 3. 南昌大学食品科学与技术国家重点实验室,江西南昌 330047;南昌大学生命科学与食品工程学院,江西南昌330047 |
| 基金项目： | 南昌市科技局农业重点科研与攻关类项目 |
| 摘 要： | 对仔猪复合微生态制剂液体发酵产酶工艺进行了优化。采用响应面分析法得到最佳培养条件为:pH6.0、发酵温度30℃、培养时间65h、接种量10%,在此培养条件下,菌体生物量理论值为2.95×1012个/ml;对最佳培养条件进行验证性试验,重复3次得到菌体生物量平均值为3.04×1012个/ml。在最优混合培养条件下研究了发酵液中的纤维素酶、淀粉酶、糖化酶的酶活性,3种酶的最大酶活力分别为1 085、492和1 192U/ml。 |
| 关 键 词： | 仔猪 微生态制剂 液体发酵工艺 酶活性 响应面分析法 饲料添加剂 |
Optimization of liquid-state fermentation technique of complex probiotics |
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| Wang Jiantao , Fu Guiming , Liu Chengmei , Wan Yin , Li Hongge , Chai Jianxin , Chen Jianfang , Hu Ming , Bai Lujie. Optimization of liquid-state fermentation technique of complex probiotics[J]. Cereal & Feed Industry, 2012, 12(1): 42-45 | |
| Authors: | Wang Jiantao Fu Guiming Liu Chengmei Wan Yin Li Hongge Chai Jianxin Chen Jianfang Hu Ming Bai Lujie |
| Affiliation: | 1,2,3 (1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;2.College of Life Science and Food Technology,Nanchang University,Nanchang 330047,China;3.Sino-German food engineering centre,Nanchang University,Nanchang 330047,China) |
| Abstract: | The liquid fermentation process of compound probiotics for piglets was optimized.Using response surface method,the optimum culture conditions were as follows: pH6.0,temperature of 30℃,incubation time of 65 h,inoculation volume of 10%;Under the conditions,the theoretical biomass reached 2.95×1012 cfu/ml.By proven test for the optimum culture conditions,the average biomass for three replicate tests was 3.04×1012 cfu/ml.Under the optimum mixed culture conditions,the activity of cellulase,amylase and glucoamylasein in the fermentation broth were studied,and the maximum enzyme activity of the three was 1 085,492 and 1 192 U/ml,respectively. |
| Keywords: | piglets probiotics liquid fermentation process enzyme activity response surface methodology feed additive |
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