高底物浓度玉米蛋白的双酶复合水解效果研究

利用碱性蛋白酶(Alcalase)、风味蛋白酶(Flavourzyme)和复合蛋白酶(Protamex)对高底物浓度(135g/L)玉米蛋白进行双酶复合水解,研究复合水解对水解物的水解度、可溶性蛋白质含量和抗氧化活性的影响,并对双酶酶解效果较好的酶解液进行了分子量分布测定。结果表明,Flavourzyme和Alcalase、Flavourzyme和Protamex、Protamex和Alcalase顺次水解玉米黄粉,总水解度分别为27.11%、26.95%和19.76%,可溶性蛋白质含量分别为50.33、40.32、48.85mg/ml,抗氧化活性分别为634.35、576.79和593.21 U/ml。多肽分子量主要分布在5 801.170～238.962u,与单酶水解相比均有显著提高。

Effect of double enzymatic hydrolysis of corn gluten of high substrate concentration

Alcalase,Flavourzyme and Protamex were used in double enzyme hydrolysis of high concentration of corn protein(135 g/L).Hydrolysis degree,soluble protein content and antioxidant activity of the hydrolysates were investigated,and molecular weight distribution of the hydrolysates under the optimum conditions were determined.The results showed that hydrolysis degree of the hydrolysates of corn gluten with Flavourzyme and Alcalase,Flavourzyme and Protamex,Protamex and Alcalase were 27.11%,26.95% and 19.76%,respectively.Soluble protein contents of the hydrolysates were 50.33,40.32 and 48.85 mg/ml,respectively.The antioxidant activities of the hydrolysates were 634.35,576.79 and 593.21 U/ml,respectively.Compared with single enzyme hydrolysis,the effects of hydrolysis with double enzyme were improved significantly.Molecular weight distribution of the hydrolysates were mainly between 5 801.170~238.962 u.