超高效液相色谱-串联四极杆飞行时间质谱法筛查饲料中11种镇静剂类药物

为了快速筛查未知饲料样品中的镇静剂类药物，应用超高效液相色谱-串联四极杆飞行时间质谱建立了筛查饲料中11种镇静剂类药物的方法。在不同添加浓度下获得了精确的分子离子质量，质量偏差的绝对值低于3×10^-6。11种镇静剂类药物在0.02~1 mg/L范围内线性关系良好，相关系数r≥0.99，添加浓度为0.5~5 mg/kg时，其添加回收率在50%~120%之间，在饲料基质中的检测限为0.1 mg/kg。该方法简便、高效、准确，适于饲料中11种镇静剂类药物的分析检测需求。

Screening Method for 11 Sedatives in Feedstuff by Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry

A method based on ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF) was developed for quantitative analysis of 11 sedatives in feedstuff.The accurate mass was obtained in different spiking levels(from 0.5 to 5 mg/kg)and the accuracy error was lower than 3×10-6,which was well within the accepted limits for target confirmation.The linearity of response ranges from 0.02 to 1.0 mg/L,and the correlation coefficient is greater than 0.99.The average recoveries range from 50% to 120% of the fortified 11 sedatives in feedstuff at 0.5—5 mg/kg levels.and the limits of detection are 0.1 mg/kg.The results indicate that the method is easy,fasty,and sensitive.This method can meet the requirements for simultaneous determination of 11 sedatives in feedstuff.