Separation of γ- and α-linolenic acid containing triacylglycerols by capillary supercritical fluid chromatography |
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Authors: | Pekka Manninen Päivi Laakso Heikki Kallio |
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Affiliation: | (1) Department of Biochemistry and Food Chemistry, University of Turku, Vatselankatu 2, 20500 Turku, Finland |
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Abstract: | The separation of γ- and α-linolenic acid containing triacylglycerols with an identical acyl carbon number and degree of unsaturation
was obtained on capillary supercritical fluid chromatography using a 25% cyanopropyl−75% methylpolysiloxane stationary phase.
The resolution of 1,3-dioleoyl-2-γ-linolenoyl-sn-glycerol and 1,3-dioleoyl-2α-linolenoyl-sn-glycerol was 1.35 on a 10 m×50 μm i.d. column, whereas the resolution was enhanced to 1.66 by combining two 10-meter columns
in series. The difference in the position of double bonds in one linolenic acid residue of triacylglycerols resulted in two
series of peaks in the separation of alpine currant (Ribes alpinum) and black currant (R. nigrum) seed oils. The use of the 10-meter column was found to be appropriate for the screening of the triacylglycerol profile in
both seed oils studied. |
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