Diferuloylputrescine and p-coumaroyl-feruloylputrescine, abundant polyamine conjugates in lipid extracts of maize kernels |
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Authors: | Moreau R A Nuñez A Singh V |
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Affiliation: | (1) Department of Agricultural Engineering, University of Illinois, 61801 Champaign, Illinois;(2) USDA, ARS, ERRC, 600 E. Mermaid Lane, 19038 Wyndmoor, PA |
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Abstract: | Extraction of corn bran or corn fiber with polar solvents such as methylene chloride, ethanol or chloroform/methanol yielded
common lipids and two unknown high-performance liquid chromatography (HPLC) peaks, each with an ultraviolet absorbance maximum
at 320 nm. HPLC-mass spectrometry revealed that the unknowns were diferuloylputrescine (DFP) and p-coumaroyl-feruloylputrescine (CFP). When compared to extracts of corn fiber (a pericarp-enriched fraction from the wet milling
of corn), comparable extracts of corn bran (a pericarp- enriched fraction from the dry milling of corn) yielded three- to
eightfold higher levels of DFP and CFP. Extraction of corn bran or fiber with an accelerated solvent extractor revealed that
elevated temperatures greatly enhanced the extraction of DFP and CFP by methylene chloride and ethanol. “Corn bran oil,” prepared
by extraction of corn bran with hot methylene chloride, contained 14 wt% DFP and 3 wt% CFP. However, when hexane was used
as a solvent, accelerated solvent extraction of the corn bran or fiber did not extract any DFP or CFP. Extraction of wheat
bran or psyllium hulls with hot methylene chloride did not yield any detectable DFP or CFP. Because it has been suggested
that polyamine conjugates such as DFP and CFP may function as natural pesticides, a rapid method was developed to purify them
so that their biological activity could be evaluated. |
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