千佛菌抗衰老作用实验研究

目的:观察千佛菌对衰老小鼠血清、脑、肝脏组织抗氧化系统的影响,探讨千佛菌抗衰老的作用机理。方法:采用腹腔注射D-半乳糖制备衰老小鼠模型,并以千佛菌100%浓度（大剂量）、50%（小剂量）浓度给小鼠灌胃治疗,六周后,处死动物。检测血清、脑、肝组织中SOD、GSH-Px、CAT的活性及MDA的含量。结果:千佛菌能显著提高血清、脑及肝组织中SOD、GSH-Px、CAT（p<0.05）活性,降低血清、肝脏和脑组织中MDA（p<0.01）含量。结论:千佛菌通过提高抗氧化酶活性,显著提高衰老小鼠的抗氧化能力,以大剂量疗效显著。 

Study on anti-aging mechanism of Grifola frondosa

Objective:To investigate the protective effect and anti -oxidative activity mechanism of Grifola frondosa in serum, heart and liver of aged mice. Methods:The aging mice were established with D-galactose by intraperitoneal injection. Grifola frondosa was administrated to mice by gavage 100% (Grifola frondosa large dose) , 50% (Grifola frondosa small dose. After six weeks, the mice were executed. The serum, heart and liver tissue of mice were collected. The content of malondialdehyde (MDA) , activity of superoxide dismutase (SOD) , glutathione peroxidase (GSH-Px) catalase (CAT) were examined by the ultraviolet spectrophotometer detection. Results:Compared with the D-galactose model group, Grifola frondosa in different doses significantly improved the SOD, CAT, GSH-Px activities (p<0.05) and reduced the MDAcontent (p<0.01) in serum, heart and brain of mice. Conclusion:Grifola frondosa possesses anti-oxidant activity to protect body against free radical-induced damage, especially for the high dose.