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| 粪肠球菌发酵生棉粕的工艺条件优化 | |
| 引用本文： | 马珍,李吕木,许发芝,丁小玲,钱坤,徐雅芫,陈丽园,张莉,陈文帮. 粪肠球菌发酵生棉粕的工艺条件优化[J]. 食品与发酵工业, 2013, 39(5): 94-99 |
| 作者姓名： | 马珍 李吕木 许发芝 丁小玲 钱坤 徐雅芫 陈丽园 张莉 陈文帮 |
| 作者单位： | 1. 安徽农业大学茶与食品科技学院,安徽合肥,230036 2. 安徽农业大学茶与食品科技学院,安徽合肥,230036;安徽农业大学动物科技学院,安徽合肥,230036 3. 安徽农业大学动物科技学院,安徽合肥,230036 4. 安徽省畜牧生物工程技术研究中心,安徽合肥,230031 5. 安徽省兽药饲料监察所,安徽合肥,230022 |
| 基金项目： | 国家"十二五"科技支撑计划，优质猪肉安全与标准化生产技术中试 |
| 摘 要： | 为降低棉粕中游离棉酚含量,对粪肠球菌固态发酵生棉粕降解游离棉酚工艺条件进行了优化。首先对影响粪肠球菌固态发酵棉粕降解游离棉酚的料水比、初始pH、发酵时间、接种量、发酵温度进行单因素研究,确定对结果有较大影响的料水比、初始pH和发酵时间3个因素。然后利用Box-Behnken设计,对其进行优化。结果表明,料水比、初始pH和发酵时间的最佳条件分别是1∶0.53、6.57和3.06d。按照优化的条件发酵棉粕后游离棉酚含量降为231.68 mg/kg,降解率达67.85%。 |
| 关 键 词： | 生棉粕 粪肠球菌 游离棉酚 固态发酵 |
Optimization of technical conditions of solid-state fermentation raw cottonseed meal with Enterococcus faecalis |
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| MA Zhen,LI Lv-mu,XU Fa-zhi,DING Xiao-ling,QIAN Kun,XU Ya-yuan,CHEN Li-yuan,ZHANG Li,CHEN Wen-bang. Optimization of technical conditions of solid-state fermentation raw cottonseed meal with Enterococcus faecalis[J]. Food and Fermentation Industries, 2013, 39(5): 94-99 | |
| Authors: | MA Zhen LI Lv-mu XU Fa-zhi DING Xiao-ling QIAN Kun XU Ya-yuan CHEN Li-yuan ZHANG Li CHEN Wen-bang |
| Affiliation: | 1(College of Tea and Food Science and Technology,Anhui Agricultural University,Hefei 230036,China) 2(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China) 3(Anhui Animal Biological Engineering Technology Research Center,Hefei 230031,China) 4(Institute of Veterinary medicine and feed,Hefei 230022,China) |
| Abstract: | To reduce content of free gossypol in cottonseed meal,this paper focused on the optimization of the condition of solid-state fermentation raw cottonseed meal with Enterococcus faecalis by response surface method.Firstly,the effects of independent variables(ratio of material to water,initial pH,fermentation time,inoculation amount and fermentation temperature) were evaluated by single factor analysis.The important factors affecting the content of free gossypol in fermentation cottonseed meal were ratio of material to water,initial pH and fermentation time.Secondly,the optimum fermentation conditions for free gossypol degradation were confirmed by Box-Behnken experimental design.The results showed that the optimum conditions were as follows: ratio of material to water was 1∶ 0.53,initial pH was 6.57,fermentation time was 3.06 days.Under the optimum condition,the content of free gossypol of fermentation cottonseed meal was 231.68mg/kg and the degradation rate of free gossypol was 67.85%. |
| Keywords: | raw cottonseed meal Enterococcus faecalis free gossypol solid-state fermentation |
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