| SARS病毒核衣壳蛋白在大肠杆菌中的表达及纯化 |
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| 引用本文： | 李洋,王宣军,张秀霞,吉海滨,王志武,方勇,盛军.SARS病毒核衣壳蛋白在大肠杆菌中的表达及纯化[J].粉末涂料与涂装,2005,18(6):465-466,469. |
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| 作者姓名： | 李洋 王宣军 张秀霞 吉海滨 王志武 方勇 盛军 |
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| 作者单位： | [1]吉林大学生命科学学院,长春130021 [2]长春生物制品研究所,长春130062 |
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| 摘 要： | 目的在大肠杆菌中高效表达SARS核衣壳蛋白。方法将已合成的SARS-CoV N蛋白基因片段,克隆至pUC-18载体,与pET28质粒连接,转化大肠杆菌,进行SARS-CoV核衣壳融合蛋白表达。用SARS患者恢复期血清进行表达产物鉴定。用色谱方法进行表达产物的层析纯化。结果SARS病毒N基因在大肠杆菌中获得了高效表达,表达量占总蛋白的30%以上;经三步纯化后纯度达90%以上。结论已获得了SARS核衣壳蛋白样品。
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| 关 键 词： | SARS病毒 核衣壳蛋白 表达 纯化 |
| 收稿时间： | 2005-06-16 |
| 修稿时间： | 2005-06-16 |
Expression of SARS-CoV Neucleocapsid Protein in E. coli and Purification of Expressed Product |
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| LI Yang, WANG Xuan-jun,ZHANG Xiu-xia,et al.Expression of SARS-CoV Neucleocapsid Protein in E. coli and Purification of Expressed Product[J].Chinese Journal of Biologicals,2005,18(6):465-466,469. |
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| Authors: | LI Yang WANG Xuan-jun ZHANG Xiu-xia |
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| Affiliation: | College of Life Science, Jilin University, Changchun 130012, China |
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| Abstract: | Objective To highly express SARS-CoV neucleocapsid protein in E. coll. Methods Insert the synthetic SARS-CoV N protein gene into vector pUC-18,ligate to plasmid pET28 and transform to E. coli for expression. Identify the expressed product by the sera of patients with SARS during recovery phase. Purify the expressed product by chromatography. Results SARS-CoV N gene was highly expressed in E. coll. The expressed product contained more than 30% of total somatic protein. The purity of expressed product after three steps of chromatography reached more than 90%. Conclusion SARS neucleocapsid protein for further study was obtained. |
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| Keywords: | SARS virus Neueleoeapsid protein Expression Purification |
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