毛樱桃籽脂溶性抗氧化成分及其体外抗自由基活性

提取了毛樱桃籽脂溶性抗氧化成分,通过考察稳定DPPH自由基(DPPH·)在517nm处吸光强度的变化测定毛樱桃籽脂溶性抗氧化物的清除自由基活性,并同Trolox进行了对比,研究结果表明,毛樱桃籽脂溶性抗氧化物具有较强的抗DPPH·能力。利用气相色谱结合质谱联用技术对该混合物组分进行了分离、分析,结果表明,该混合物主要由γ-谷甾醇、角鲨烯、γ-生育酚、愈创醇、古巴烯醇、视黄酸甲酯组成,这些成分的协同作用贡献于毛樱桃籽脂溶性抗氧化物的强抗DPPH·活性。毛樱桃籽脂溶性抗氧化物对于毛樱桃籽脂质及其副产物的深加工和开发应用具有重要的意义。

Constituents and Anti-oxidizing Properties of Lipid Fractions from Prunus tomentosa Thumb Seed in vitro

The radical scavenging potential of lipidic fractions from Prunus tomentosa Thumb seed was evaluated for their capacity to scavenge the relatively stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) by assaying the change of absorbance of the free radical solution at 517nm, with data obtained compared with Trolox. Results showed that there is potent anti- oxidizing activity in the test system. Anti-oxidizing constituents are characterized by capillary gas chromatography coupled with mass spectrometry(GC/MS) detection to be composed of gamma-sitosterol, squalene, retinoic acid methyl ester, gamma- tocopherol, champacol and cis-alpha-copaene-8-ol. They are the most important natural anti-oxidizing contained in Prunus tomentosa Thumb seed oil , with attribution to the DPPH radical-scavenging and the anti-oxidizing potential as a result of the combination of such specific components. Data obtained also suggest that the oil possesses biological properties that could partially be beneficial to health as synergic effects of diet, the information is of importance in processing and utilizing the crude oils and theirb yproducts.