| 豆科植物不同发育阶段不同部位乙酰胆碱酯酶活性研究 |
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| 引用本文： | 纪淑娟,刘艳平,白冰,蒋东华,张旋,那其朔.豆科植物不同发育阶段不同部位乙酰胆碱酯酶活性研究[J].食品工业科技,2010(4). |
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| 作者姓名： | 纪淑娟 刘艳平 白冰 蒋东华 张旋 那其朔 |
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| 作者单位： | 沈阳农业大学食品学院,辽宁沈阳,110161 |
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| 基金项目： | 辽宁省自然科学基金项目(20082125) |
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| 摘 要： | 采用比色法研究不同种类豆科植物于不同发育阶段、不同部位乙酰胆碱酯酶(AChE)活性,并测定其总酯酶活性、蛋白质含量、水分含量,旨在为农药残留快速检测提供配套的新酶原。结果表明,在不同种类的豆科植物中均检测到乙酰胆碱酯酶活性,但不同种类豆科植物存在差异,干种子期、幼芽期及幼苗期各部位的乙酰胆碱酯酶活性存在差异,以每克干物质中乙酰胆碱酯酶酶活计,干种子期猪腰豆(Whitfordiodendron filipes Dunn)中乙酰胆碱酯酶酶活性最高,为10.28U/g干重。
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| 关 键 词： | 豆科植物 发育阶段 乙酰胆碱酯酶 活性 有机磷农药 酶原 |
Acetylcholinesterase activity for leguminous plant at different developmental stages and different parts |
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| JI Shu-juan,LIU Yan-ping,BAI Bing,JIANG Dong-hua,ZHANG Xuan,NA Qi-shuo.Acetylcholinesterase activity for leguminous plant at different developmental stages and different parts[J].Science and Technology of Food Industry,2010(4). |
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| Authors: | JI Shu-juan LIU Yan-ping BAI Bing JIANG Dong-hua ZHANG Xuan NA Qi-shuo |
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| Affiliation: | JI Shu-juan,LIU Yan-ping,BAI Bing,JIANG Dong-hua,ZHANG Xuan,NA Qi-shuo(College of Food Science,Shenyang Agriculture University,Shenyang 110161,China) |
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| Abstract: | In order to develop new source of enzyme utilized for rapid detection of pesticide residue in agricultural product,the activity of acetylcholinesterase (AChE) in different kinds of leguminous plants at different parts and different development stages were detected by the colorimetry method. The total esterase activity,the content of protein and water were tested simultaneously. Results showed that AChE activities were detectable in various leguminous plants. The differences are evident among different kinds... |
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| Keywords: | leguminous plant development stage acetylcholinesterase activity pesticide residue zymogen |
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