Modeling and optimization of bioactive compounds from chickpea seeds (Cicer arietinum L) |
| |
| Authors: | Ivan M. Savic Ivana Lj. Nikolic Ivana M. Savic-Gajic Tatjana D. Kundakovic |
| |
| Affiliation: | 1. Department of Organic-technological Sciences, Faculty of Technology, University of Nis, Leskovac, Serbia;2. Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, Belgrade, Republic of Serbia |
| |
| Abstract: | The aim of study was to model and optimize the extraction of polyphenols and flavonoids with expressed antioxidant activity from chickpea seeds using a central composite design. The optimal extraction conditions were the extraction time of 145.5 min, ethanol concentration of 83.7% and liquid-to-solid ratio of 24.9 (expressed as cm3 per g). The content of total polyphenol and flavonoid was 2.75 g gallic acid equivalent per kilogram of the dried extract and 0.147 g rutin equivalent per kilogram of the dried extract, respectively. Using 2,2-diphenyl-1-picrylhydrazyl assay, it was found that the optimal extract had the IC50 of 1.55 mg cm?3. |
| |
| Keywords: | Chickpea total polyphenol total flavonoid antioxidant activity central composite design |
|
|
