|
|||||
|
|
Rapid screening and separating two radical scavengers in Lycium barbarum L. by DPPH-HPLC analysis-combined dual-mode high-speed countercurrent chromatography |
|
| Authors: | Wen-Da Duan Gao-Hong Wang Xiao-Feng Liu Dong Pei Jian-Fei Liu |
| Affiliation: | 1. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory of Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China;2. University of the Chinese Academy of Sciences, Beijing, China;3. College of Life science and Engineering, Lanzhou University of Technology, Lanzhou, China |
| Abstract: | A target-guidance separation strategy composed of activity screening process with high-performance liquid chromatography and separating process with high-speed countercurrent chromatography (HSCCC) has been developed and used to screen and separate radical scavengers from Lycium barbarum extract. Two compounds were found to be radical scavengers and dual-mode HSCCC was used to separate these two defined compounds due to the big gap in polarity between them. Rutin and quercetin were separated with purities of 96.5% and 95.0%. Their structures were identified by nuclear magnetic resonance and both of them exhibit potent radical scavenging activity with the IC50 values being 20.07 ± 0.10 and 3.11 ± 0.03 μg/mL, respectively. |
| Keywords: | Radical scavenger Lycium barbarum L high-speed countercurrent chromatography dual-mode elution |