Continuous Hydrolysis of Fructans in Jerusalem Artichoke Extracts using Immobilized Nonviable Cells of Kluyveromyces marxianus |
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| Authors: | S R PAREKH A MARGARITIS |
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| Affiliation: | Author Margaritis is with the Chemical &Biochemical Engineering Faculty of Engineering Science, The Univ. of Western Ontario, London, Ontario Canada N6A 5B9. Author Parekh's present address is Dept. of Chemical Engineering &Applied Chemistry, Univ. of Toronto, Toronto, Ontario Canada M53 1A4. |
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| Abstract: | Non-viable immobilized cells of Kluyveromyces marxianus in alginate beads having inulase (ß-2,1-fructan fructano-hydrolyase E.C.3.2.1.7) acitivity were used as biocatalyst in a packed bed reactor. Extracts of Jerusalem artichoke tubers were contacted with the biocatalyst for continuous conversion of the fructan component to fructose. In a bed reactor packed with 100 mL of beads, a volumetric productivity of 136 g/L/hr total reducing sugars was obtained with 98% substrate conversion. When operated continuously for 30 days, a 55% loss in the original activity was observed, giving a half life for the biocatalyst of 28 days. |
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