The accumulation of P-element-induced recombinants in the germline of male Drosophila melanogaster |
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Authors: | MM Tanaka XM Liang YH Gray JA Sved |
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Affiliation: | School of Biological Sciences, Sydney University, NSW, Australia. markt@charles.stanford.edu |
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Abstract: | P-element-induced recombination in Drosophila melanogaster occurs premeiotically. Recombinants are therefore expected to accumulate in the stem cells of the germline of P-element-carrying males. We show that both the recombination frequency and the incidence of "clustering" increase with the age of males carrying various P-element derivatives. The combination of end-deleted elements can lead to average recombination frequencies >50% with individual instances of 100% recombination. These elements also lowered the fertility of the carriers. We investigated these features by constructing an analytical and a computer simulation model of the course of events in the germline, incorporating the recently proposed hybrid element insertion (HEI) model of P-element activity. The model is able to predict extreme recombination levels, segregation ratio biases and lowered fertility through cell death in a single analysis. |
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