| 用改良的离子交换层析法分离高纯度凝血因子Ⅷ |
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| 引用本文： | 邹金森 穆海燕. 用改良的离子交换层析法分离高纯度凝血因子Ⅷ[J]. 中国生物制品学杂志, 1994, 7(2): 64-66 |
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| 作者姓名： | 邹金森 穆海燕 |
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| 作者单位： | 卫生部武汉生物制品研究所!武昌，430060，卫生部武汉生物制品研究所!武昌，430060，卫生部武汉生物制品研究所!武昌，430060，卫生部武汉生物制品研究所!武昌，430060，卫生部武汉生物制品研究所!武昌，430060，卫生部武汉生物制品研究所!武昌，430060 |
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| 摘 要： | 应用改良的DEAE－Fractogel阴离子交换法生产高纯度FⅧ，并经过TNBP和Tween－80灭活病毒，使单位体积凝胶交换容量增加20％，并改进了冻干制品的溶解性和稳定性，FⅧ活性保持30IU／ml以上，比活性达到200IU／ml蛋白，安全性可靠，适合于大规模生产。
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| 关 键 词： | 高纯度Ⅷ因子 离子交换 病毒灭活 |
Seperation of Highly Purified FⅧ Concentrate By Improved Ion-Exchange Chromatography |
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| Zou Jinsen et al. Seperation of Highly Purified FⅧ Concentrate By Improved Ion-Exchange Chromatography[J]. Chinese Journal of Bilogicals, 1994, 7(2): 64-66 |
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| Authors: | Zou Jinsen et al |
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| Abstract: | Highly purified FⅧ concentrate was seperated by improved DEAE-Fractogel anion exchange chromatography. By inactiving virus with TNBP and Tween-80, the exchange capacity of unit volumn of gel increased by 20%, and the solubility and stability of freeze-dried final products werc also improved. The activity of FⅧ: C was above 30IU/ml,and the specific activity of it reached 200IU/mg protein. The FⅧ: C seperated by this method also showed reliable safety. This study suggested that the method was suitable for large-scale production. |
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| Keywords: | Highly purified FⅧ: C Ion-exchange Virus inactivation |
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