| 紫外分光光度法测定含乳饮料中游离氨基酸含量 |
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| 引用本文： | 胡京枝,董小海,余大杰.紫外分光光度法测定含乳饮料中游离氨基酸含量[J].中国食品添加剂,2007(6):164-166,64. |
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| 作者姓名： | 胡京枝 董小海 余大杰 |
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| 作者单位： | 河南省农业科学院农业质量标准与检测技术研究中心,郑州,450002 |
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| 摘 要： | 牛奶中游离氨基酸主要是谷氨酸、甘氨酸,其含量在一定的范围内;笔者对含乳饮料中游离氨基酸的含量的试验条件进行了研究,建立了紫外分光光度法测定游离氨基酸含量的测定方法。结果表明乙酸锌和亚铁氰化钾溶液作沉淀剂,沉淀效果较好,用乙二醇作茚三酮的溶剂比乙二醇独甲醚的稳定性好,方法具有良好的回收率和重复性。
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| 关 键 词： | 含乳饮料 游离氨基酸 茚三酮 紫外分光光度法 |
| 文章编号： | 1006-2513（2007）06-0164-03 |
| 收稿时间： | 2007-08-20 |
| 修稿时间： | 2007年8月20日 |
Determination method of free amino acid content in drink milk by UV-spectrometer |
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| HU Jing-zhi,DONG Xiao-hai,YU Da-hai.Determination method of free amino acid content in drink milk by UV-spectrometer[J].China Food Additives,2007(6):164-166,64. |
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| Authors: | HU Jing-zhi DONG Xiao-hai YU Da-hai |
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| Abstract: | The free amino acid content in milk are mostly glutanic acid,glycine acid,the content are certainty;This study of experiment condition to the free amino acid content in drink milk,the method of determining free amino acid in drink milk was established using ultraviolet spectrophotometry.The result indicated that Zinc acetate and Potassium ferrocyanide trihydrate as precipitating reagent,the effection were better.The stability of ethylene glycol as solvent of triketone was better than ethylene glycol monomethyl ether,the method indicated good rate of recover and duplication. |
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| Keywords: | drink milk free amino acid triketone UV-spectrometer |
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