响应曲面法优化山蕗菜根多糖的提取工艺

以山蕗菜根提取类黄酮后的残渣为材料，研究山蕗菜根多糖的提取工艺。在单因素试验基础上，选定提取温度、时间和料液比3 个因素的3 个水平进行中心组合试验，建立多糖提取率的二次回归方程，通过响应面分析得到优化组合条件。结果表明：提取工艺条件为提取温度70℃、提取时间2.3h、料液比1:45(g/mL)、提取1 次时，多糖提取率达到最大值。该条件下多糖提取率预测值为35.04%，验证值为34.74%。

Optimization of Extraction Technology of Polysaccharides from Petasites tricholobus Roots by Response Surface Analysis

Crude polysaccharides were prepared from the residue of Petasites tricholobus roots after flavonoids extraction. According to single-factor experiments, three levels of the factors including extraction temperature, extraction time and liquid/solid ratio were selected for the central composite design to establish a quadric regression equation for predicting the yield of polysaccharides. By response surface analysis, the optimum extraction conditions were obtained as follows: extraction temperature 70 ℃, extraction time 2.3 h and liquid/solid ratio 45:1, which led to estimated and observed values of maximal yield of polysaccharides of 35.04% and 34.74%, respectively.