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Simultaneous purification and site-specific modification of pyrroline-carboxy-lysine proteins |
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| Authors: | Chiu Hsien-Po Grünewald Jan Hao Xueshi Brock Ansgar Okach Linda Uno Tetsuo Geierstanger Bernhard H |
| Affiliation: | Genomics Institute of the Novartis Research Foundation, 10675 John-Jay-Hopkins Drive, San Diego, CA 92121, USA. |
| Abstract: | Sticky residue: Pyrroline-carboxy-lysine (Pcl) can be readily incorporated into proteins expressed in E. coli and mammalian cells by using the pyrrolysyl tRNA/tRNA synthetase pair. Pcl can be used as a single amino acid purification tag and can be site-specifically modified with functional probes during the elution process. |
| Keywords: | affinity tags protein engineering protein expression protein purification pyrroline‐carboxy‐lysine |
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