In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells |
| |
Authors: | Osvaldo Mercuri María Elena De Tomás Roberto J de Antueno |
| |
Affiliation: | (1) Cátedra de Bioquimica, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 1900 La Plata, Argentina;(2) Present address: Carrera del Investigador Cientifico, Consejo Nacional de Investigaciones Cientificas y Técnicas, Argentina |
| |
Abstract: | In this paper, evidence is presented on the capacity of Ehrlich ascites cells to synthesize in vitro monounsaturated fatty
acids from radioactive palmitate. Localization of the double bond was determined by ozonolysis and subsequent reduction of
the ozonides to aldesters followed by gas liquid chromatography. These results proved that Ehrlich ascites cells have a Δ9
desaturase that catalyzes the biosynthesis of palmitoleic acid from palmitic acid and oleic and vaccenic acid via elongation-desaturation
and desaturation-elongation, respectively, using palmitic acid as substrate. Furthermore, it is shown that, as in the hepatic
cells, Δ9 desaturase enzyme activity of the tumoral cells is associated with the endoplasmic reticulum. The electron transport
components involved in the desaturase system, i.e., NADH-cytochrome bs reductase and NADH-cytochrome c reductase, were also measured. The activities of these enzymes do not appear to be rate-limiting
in the desaturase activity of these tumoral cells. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|