| 利用生化方法鉴定灵芝原生质体融合子的研究 |
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| 引用本文： | 苗敬芝,朱芹波,吕兆启,董玉玮,曹泽虹. 利用生化方法鉴定灵芝原生质体融合子的研究[J]. 食品科技, 2010, 0(4) |
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| 作者姓名： | 苗敬芝 朱芹波 吕兆启 董玉玮 曹泽虹 |
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| 作者单位： | 徐州工程学院食品工程学院; |
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| 基金项目： | 江苏省高校自然科学基础研究项目(08KJD18002) |
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| 摘 要： | 以美大灵芝和信州灵芝为出发菌株,采用化学融合法将两种灵芝原生质体进行融合,利用生化方法进行检测。结果表明:融合子胞外多糖含量与美大和信芝两亲本相比分别增加了332.80%和22.86%,胞内三萜含量与美大和信芝相比分别增加了36.21%和113.16%,胞外三萜含量与美大和信芝相比分别增加了23.17%和46.31%;采用琼脂糖凝胶电泳实验对融合子及亲本DNA进行检测,融合子DNA移动速度比亲本慢,说明融合子分子量大于两亲本,两亲本遗传物质发生重组。
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| 关 键 词： | 灵芝 原生质体融合 融合子 生化方法 鉴定 |
Research on protoplast fusant of Ganoderma lucidum using biochemistry identification method |
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| MIAO Jing-zhi,ZHU Qin-bo,LV Zhao-qi,DONG Yu-wei,CAO Ze-hong. Research on protoplast fusant of Ganoderma lucidum using biochemistry identification method[J]. Food Science and Technology, 2010, 0(4) |
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| Authors: | MIAO Jing-zhi ZHU Qin-bo LV Zhao-qi DONG Yu-wei CAO Ze-hong |
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| Affiliation: | MIAO Jing-zhi,ZHU Qin-bo,LV Zhao-qi,DONG Yu-wei,CAO Ze-hong(Food Institute of Technology,Xuzhou Institute of Technology,Xuzhou 221008) |
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| Abstract: | Ganoderma lucidum Meida and the Ganoderma lucidum Xinzhi were used as starting strains and their protoplasts were fused by chemistry method,biochemistry identification method was used to detect the results in this paper.The results showed that extracellular polysaccharide content of fusant was increased 332.80% and 22.86% compared with Ganoderma lucidum Meida and Ganoderma lucidum Xinzhi separatedly,intracellular triterpenoid content was increased 36.21% and 113.16% compared with Ganoderma lucidum Meida and... |
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| Keywords: | Ganoderma lucidum protoplast fusion fusant biochemical methods identification |
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