南瓜籽多肽的制备及其对人体皮肤细胞的体外作用

以南瓜籽为原料制备南瓜籽蛋白。分别采用碱性蛋白酶、胰蛋白酶、风味蛋白酶、碱性蛋白酶-胰蛋白酶、碱性蛋白酶-风味蛋白酶酶解南瓜籽蛋白得到南瓜籽多肽。结果表明:碱性蛋白酶-胰蛋白酶酶解南瓜籽蛋白,水解度为27. 23%、多肽产率为39. 20%,均为5种酶解产物中最高;碱性蛋白酶-胰蛋白酶酶解制备的南瓜籽多肽中小于1 000 Da的多肽含量高达92. 72%,其具有较好的促进人体皮肤细胞增殖的性能及清除自由基作用,可降低细胞内活性氧含量,在人体皮肤细胞受到氧化损伤时,能起到良好的修复作用。

Preparation of pumpkin seed polypeptide and its effects on human skin cells in vitro

Using pumpkin seeds as raw materials, pumpkin seed protein was prepared. Five polypeptides were prepared respectively by enzymatic hydrolysis of pumpkin seed protein with alkaline protease, trypsin, flavor protease, alkaline protease-trypsin and alkaline protease-flavor protease. The results showed that among the five hydrolysates, the degree of hydrolysis (DH) and the yield of polypeptide with alkaline protease-trypsin were the highest (the DH value 27.23% and the yield of polypeptide 39.20%). Because the content of less than 1 000 Da polypeptide in the pumpkin seed polypeptide prepared by alkaline protease-trypsin was as high as 92.72%, so it had a good performance in promoting human skin cell proliferation. In addition, it could scavenge free radicals and reduce the content of intracellular reactive oxygen species, so it could repair cells when human skin cells were damaged by oxidation.