| 酶法催化酯交换制备甘油二酯工艺优化研究 |
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| 引用本文： | 马靖轩马传国李利君刘 伟刘 君. 酶法催化酯交换制备甘油二酯工艺优化研究[J]. 中国油脂, 2020, 45(1): 51-55 |
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| 作者姓名： | 马靖轩马传国李利君刘 伟刘 君 |
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| 作者单位： | 1.河南工业大学 粮油食品学院，郑州 450001； 2.小麦和玉米深加工国家工程实验室,郑州 450001；3.国家粮食局粮油食品工程技术研究中心，郑州 450001 |
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| 基金项目： | “十三五”国家重点研发计划（2016YFD0401404）；河南省科技厅自然科学项目（152100210265） |
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| 摘 要： | 以大豆油和单甘酯为原料,在无溶剂体系中利用固定化脂肪酶Novozym 435催化合成甘油二酯。通过单因素实验和响应面实验研究反应温度、底物摩尔比、反应时间和酶添加量对甘油二酯含量的影响。结果表明:4个因素对甘油二酯含量影响的大小依次为反应温度、反应时间、酶添加量、底物摩尔比;合成甘油二酯的最佳工艺条件为大豆油与单甘酯摩尔比1∶2、酶添加量9%、反应温度83℃、反应时间6. 5 h,在此条件下甘油二酯含量为(51. 2±0. 2)%。
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| 关 键 词： | 甘油二酯 大豆油 单甘酯 酯交换 固定化脂肪酶 |
Optimization of enzymatic transesterification to prepare diglyceride |
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| MA JingxuanMA ChuanguoLI LijunLIU WeiLIU Jun. Optimization of enzymatic transesterification to prepare diglyceride[J]. China Oils and Fats, 2020, 45(1): 51-55 |
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| Authors: | MA JingxuanMA ChuanguoLI LijunLIU WeiLIU Jun |
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| Affiliation: | (College of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China;National Engineering Laboratory for Wheat and Com Further Processing,Zhengzhou 450001,China;Grain and Oil Food Engineering Technology Research Center,State Administration of Grain,Zhengzhou 450001,China) |
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| Abstract: | Diglyceride was synthesized from soybean oil and monoglyceride by immobilized Novozym 435 in a solvent-free system. The effects of reaction temperature,reaction time,enzyme dosage,and substrate molar ratio on the diglyceride content were studied by single factor experiment and response surface methodology. The results showed that the effect of reaction temperature on the diglyceride content was the greatest,followed by reaction time,enzyme dosage and substrate molar ratio. The optimal conditions were obtained as follows: molar ratio of soybean oil to monoglyceride 1∶ 2,enzyme dosage 9%,reaction temperature 83 ℃ and reaction time 6. 5 h. Under the optimal conditions,the diglyceride content was( 51. 2 ± 0. 2) %. |
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| Keywords: | diglyceride soybean oil monoglyceride transesterification immobilized lipase |
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