碳酸氢钾和氯化钠对烟草转化株烟碱转化的诱导作用

以白肋烟TN90高转化株为材料研究了无机盐处理烟株早期叶片对烟碱转化的诱导作用及机理。结果表明,1.2%的碳酸氢钾水溶液和1.2%的氯化钠水溶液均具有显著的诱导转化株早期叶片烟碱转化作用,碳酸钠效果较差。比较碳酸氢钾处理、氯化钠处理分别对常规TN90品种烟株转化株的早期鉴定结果和自然晾制后烟叶的烟碱转化率发现,采用2种无机盐鉴别出的转化株均包括了所有在自然晾制后产生的转化株,且还可鉴别出一些在正常晾制条件下不能有效鉴别的低转化株;早期测定的烟碱转化率一般高于烟叶自然晾制后的烟碱转化率,有利于提高鉴定的准确性和有效性。碳酸氢钾可显著促进叶片内源乙烯的释放,在处理溶液中添加10-4mol/LAVG抑制乙烯的合成可逆转碳酸氢钾对烟碱转化的诱导。

Stimulating effect of KHCO3 and NaCl on nicotine conversion of burley converters

Burley variety TN90 converters were used to investigate the stimulating effect of inorganic salts on nicotine conversion of fresh leaves and the stimulating mechanism. It has been showed that the 1.2% potassium bicarbonate and 1.2% sodium chloride aqueous solutions both had significant stimulating effect on nicotine conversion of early stage converter leaves. Sodium carbonate did't show much stimulating effect. Comparing the early identification results achieved by using potassium bicarbonate and sodium chloride solution with the identification results after air-curing, we found that the early identified converter by potassium bicabonate and sodium chloride matched all the converters identified after normal air-curing, and the method was able to identify some minor converters which were hard to be identified normally. The percent nicotine conversions obtained at early identification were higher than that measured after air-curing, which was conductive to increasing the accuracy and availability of identification. Potasssium bicarbonate significantly increased the production and release of endogenous ethylene. The addition of 10-4M AVG (an ethylene producing inhibitor) to the potassium bicarbonate solution reversed the stimulation of nicotine conversion resulting in the conversion level comparable to control (pure water).